Anti-PAX3 Antibody Picoband™

Product Name

Anti-PAX3 Antibody Picoband™

View all PAX3 Antibodies

SKU/Catalog Number

A00285-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PAX3 Antibody Picoband™ catalog # A03534-1. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PAX3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00285-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human PAX3 recombinant protein (Position: Q306-E420). Human PAX3 shares 99.1% amino acid (aa) sequence identity with mouse PAX3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00285-2 is reactive to PAX3 in Human

Applications

A00285-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

69 kDa

Calculated molecular weight

52.968kDa

Background of PAX3

The PAX3 (paired box gene 3) gene encodes a member of the paired box or PAX family of transcription factors. This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PAX3 using anti-PAX3 antibody (A00285-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A375 whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX3 antigen affinity purified polyclonal antibody (Catalog # A00285-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX3 at approximately 69 kDa. The expected band size for PAX3 is at 53 kDa.

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Figure 2. IF analysis of PAX3 using anti-PAX3 antibody (A00285-2) and anti-Beta Tubulin antibody (M01857-3).
PAX3 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PAX3 Antibody (A00285-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For PAX3 (Source: Uniprot.org, NCBI)

Alternative Names

CDHS; HUP2; HUP2MGC120384; MGC120381; MGC120382; MGC120383; MGC134778; paired box 3; paired box gene 3 (Waardenburg syndrome 1); paired box homeotic gene 3; paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; Pax3; Waardenburg syndrome 1; WS1; WS3 PAX3 CDHS, HUP2, WS1, WS3 paired box 3 paired box protein Pax-3|paired box homeotic gene 3|paired domain gene 3|paired domain gene HuP2|transcriptional factor PAX3

For more info on PAX3, check out the PAX3 Infographic

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In this infographic, you will see the following information for PAX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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